SubtiBank SubtiBank
ykuK [2019-02-19 19:42:30]
You are currently viewing an outdated version of SubtiWiki. Please use the newest version!

ykuK [2019-02-19 19:42:30]

unknown
locus
BSU14110
pI
6.42
mw
19.34 kDa
protein length
172 aa Sequence Blast
gene length
519 bp Sequence Blast
function
unknown
product
unknown, distantly related to RNase H
essential
no
synonyms
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 6|Groups of genes] → [category|SW 6.7|Proteins of unknown function]

    • Gene

    Coordinates
    1,484,466 1,484,984

    The protein

    [SW|Domains]

  • [http://www.ncbi.nlm.nih.gov/Structure/cdd/cddsrv.cgi?ascbin=8&maxaln=10&seltype=2&uid=pfam04308 DUF458]

    • Structure

  • [PDB|2E3U] (from Pyrococcus horikoshii, 24% identity) [pubmed|17654551]

    • Expression and Regulation

    Operons

    genes
    [gene|A26502ADAC214D45A8F7F394587B0BDFBE7AF28E|ykuJ]-[gene|7FD784C885605F7FD27898BC9E83C28F21BE0E9D|ykuK]-[gene|899FF5BEE5D3F01778A0175E293EDBBDEB25717D|abbA]-[gene|90AFF93D6E7BC993B2773C12EE400C80A87A4831|darB]-[gene|FE15A41A58A8177280817CA3825764C39185021A|ccpC]
    description
    [Pubmed|16395550]

    regulatory mechanism

  • [protein|2C54FE2ADC82FF414D732018C90649D477A925AD|Spo0A]: activation, by [protein|2C54FE2ADC82FF414D732018C90649D477A925AD|Spo0A]~P [Pubmed|18840696,14651647], in [regulon|2C54FE2ADC82FF414D732018C90649D477A925AD|Spo0A regulon]
  • [protein|E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|CcpA]: repression, [Pubmed|16395550,12850135], in [regulon|E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|CcpA regulon]

    • regulation

  • expressed under conditions that trigger sporulation ([SW|Spo0A]) [Pubmed|14651647]
    • view in new tab

    Biological materials

    Mutant

  • MGNA-A772 (ykuK::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/772 NBRP B. subtilis, Japan]
  • GP214 (deletion of ''[gene|A26502ADAC214D45A8F7F394587B0BDFBE7AF28E|ykuJ]-[gene|7FD784C885605F7FD27898BC9E83C28F21BE0E9D|ykuK]-[gene|899FF5BEE5D3F01778A0175E293EDBBDEB25717D|abbA]-[gene|90AFF93D6E7BC993B2773C12EE400C80A87A4831|darB]'', replaced by ''aphA3''), available in the [SW|Stlke] lab
  • BKE14110 ([gene|7FD784C885605F7FD27898BC9E83C28F21BE0E9D|ykuK]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE14110 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCACGACACTCCTTTTCA, downstream forward: _UP4_TAATTTGGCTGAAAAAGCCA
  • BKK14110 ([gene|7FD784C885605F7FD27898BC9E83C28F21BE0E9D|ykuK]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK14110 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCACGACACTCCTTTTCA, downstream forward: _UP4_TAATTTGGCTGAAAAAGCCA

    • lacZ fusion

  • pGP709 (in [protein|search|pAC5]), available in [SW|Stlke] lab, pGP713 (in [protein|search|pAC6]), available in [SW|Stlke] lab

    • References

  • 16395550,16165328,17654551